Abstract
P311 was identified to markedly promote cutaneous wound healing by our group. Angiogenesis plays a key role in wound healing. In this study, we sought to define the role of P311 in skin wound angiogenesis. It was noted that P311 was expressed in endothelial cells in the dermis of murine and human skin wounds. The expression of P311 was confirmed in cultured murine dermal microvascular endothelial cells (mDMECs). Moreover, it was found that knockout of P311 could attenuate the formation of tubes and motility of mDMECs significantly in vitro. In the subcutaneous Matrigel implant model, the angiogenesis was reduced significantly in P311 knockout mice. In addition, wound healing was delayed in P311 knockout mice compared with that in the wild type. Granulation tissue formation during the defective wound healing showed thinner and blood vessel numbers in wound areas in P311 knockout mice were decreased significantly. A reduction in VEGF and TGFβ1 was also found in P311 KO mice wounds, which implied that P311 may modulate the exprssion of VEGF and TGFβ1 in wound healing. Together, our findings suggest that P311 plays an important role in angiogenesis in wound healing.
Highlights
P311 is a highly conserved 8-kDa intracellular protein containing 68 amino acids (Yao et al, 2017)
We found that P311 could promote cutaneous wound healing through increasing the activity of RhoA and Rac1 (Yao et al, 2017) and inducing EpMyT (Epidermal stem cell transdifferentiate into myofibroblasts; Li et al, 2016)
We carried out experiments to determine whether P311 may have a function in angiogenesis in wound healing
Summary
P311 ( known as PTZ17, Neuronal protein 3.1) is a highly conserved 8-kDa intracellular protein containing 68 amino acids (Yao et al, 2017). Following firstly identified to expressed in the embryonic brain in mice by Studler (Studler et al, 1993), P311 was found to express in almost all kinds of cells, like motoneurons (Fujitani et al, 2004), kidney tubular epithelial cells (Yao et al, 2015), glioblastomas (McDonough et al, 2005), epidermal stem cells (Li et al, 2016), fibroblast (Tan et al, 2010; Cheng et al, 2017), smooth muscle cells (Badri et al, 2013), which implied wide biological functions of P311. P311 was identified as a inducer of EpMyT $(Epidermal stem cell transdifferentiate into myofibroblasts) through TGFβ1/Smad signaling (Li et al, 2016)
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