Abstract
Bolus i.v. administration of the fast and slow H 2 S donors, Na 2 S and AP39 (2–30 μM/kg), transiently decreased rat blood pressure, heart rate and significantly influenced +dP/dt max and position of a dicrotic notch. To study an involvement of membrane channels in the effects, we compared an influence of the H 2 S donors on electrical properties of the rat heart ryanodine calcium channel (RyR2) and on the Ca-v3.1 and Ca-v3.3 T-type voltage dependent calcium channels (VDCC) expressed in HEK293 cells. AP39 (10–20 μM) inhibited RyR2 channels from the cytoplasmic side, but not from the luminal side. Effects of Na 2 S were more complex; it activated the channel at 50 μM concentration, indicating that the fast and slow H 2 S donors had different effects on RyR2. AP39 (0.3–10 μM) inhibited calcium current of Ca-v3.1 and Ca-v3.3 VDCC (from 33% to 81%) at the concentration dependent manner. Since AP39 influenced calcium channels and rat hemodynamic parameters at similar concentrations, we assume that the H 2 S-channel interaction may contribute to the donor’s cardiovascular effects.
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