Abstract
A neuron has axon and somata which show distinctly different functions. For this reason, compartmental culture of neurons has been studied as a useful research tool for axonal pathology. Primary neurons, which have been cultured in compartmental microfluidic devices, are limited to be prepared in time and location. In contrast, embryonic stem cells (ESCs) have abilities of self-renewal without limit and differentiation toward neurons and thus, they can be a substitute to overcome the limitation of primary neurons. In this research, we successfully differentiated mouse ESCs to neurons in a compartmental microfluidic device and isolated axons apart from the cell bodies. This system can be a useful platform for axonal biology researches, instead of primary neuron platform.
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