P3 Trichilia monadelpha (TM) Thonn J.J. de Wilde ameliorates oxidative stress and inflammation in TNBS-induced colitis

Abstract

Trichilia monadelpha Thonn J.J. de Wilde (TM) is a plant commonly used for the treatment of gastrointestinal disorders, inflammatory diseases and pain. This study evaluated the beneficial effect of the aqueous-methanol extract of stem bark of TM in rat colitis induced by trinitrobenzenesulfonic acid (TNBS). Thirty male Wistar rats weighing 180–200 g were randomly allocated to six groups of 5 rats; non-colitic, untreated colitic, colitic treated with prednisolone (2 mg/kg) or colitic rats treated TM (100–400 mg/kg). Rats were treated with TM orally for two days prior to colitis induction and thereafter for 7 days. Response to treatment was assessed both macroscopically and biochemically. Treatment with prednisolone and TM significantly reduced the colonic damage score, weight/length ratio and leucocyte infiltration indicated by decreased colonic myeloperoxidase activity (MPO) activity when compared to the untreated colitic animals ( P ⩽ 0.05). Furthermore, TM at 200 and 400 mg/kg doses and prednisolone (2 mg/kg) significantly prevented the depletion of colonic total glutathione content (GSH) content levels in colitic rats ( P Trichilia monadelpha ameliorates oxidative stress and inflammation in colitic rats. The beneficial effect can be further explored.