P3.12-004 Maidong on the Prevention and Treatment of Radiation Pneumonitis in Mice C57BL/6

Abstract

Radiation pneumonia is the most serious complications after radiotherapy of thoracic tumor. We use the Maidong extract as essential prescription, to assess its prevention and control effect of radiation pneumonia, and to explore its possible mechanism. 60 C57BL/6 mice were divided into:(1) blank control group, (2)merely irradiated group, (3)Chinese medicine (Maidong) group, and (4)western medicine (dexamethasone combine cefalexin)group, each group of 15 mice. Maidong extract was given 1 times per day to the mice of Chinese medicine group, dexamethasone and cefalexin were given 1 times per day to the mice of western medicine group by intragastric administration from 4 days prior to the irradiation day, last for 2 weeks; merely irradiated group and blank control group were given physiological saline instead. In addition to the blank control group, other groups were given chest a 6MV-X-ray single-wide irradiation of 18Gy.Each group randomly sacrificed 5 mice at 24 hours, 4 weeks, 12 weeks after irradiation. Took the blood, left lungs into homogenate and fixed right lungs in formaldehyde solution for research. 1, Compared to the merely irradiated group, the level of plasma IL-6(P<0.01), TNF-α(P<0.05)and TGF-β1(P<0.01), hydroxyproline(P<0.05) and MDA (P<0.01)content of the lung tissue in the Chinese medicine group mice were significantly reduced, SOD activity was significantly enhanced (P<0.01),and the expression of MMP-2(P<0.01) and TIMP-2(P<0.05) proteins were significantly reduced. 2, Compared to the western medicine group, the level of plasma IL-6, hydroxyproline content and SOD activity of the lung tissue, the expression of TIMP-2 protein didn’t show significant difference(p>0.05); because of the increase of the level of plasma TNF-α and TGF-β1(P<0.05), MDA (P<0.01)content of the lung tissue in western medicine group mice, the Chinese medicine group were significantly different at 12week; the expression of MMP-2(P<0.05) protein the Chinese medicine group were significantly higher than western medicine group at 4week, but didn’t show significant difference at 12week. Maidong has certain effect on the prevention and control of radiation pneumonia, the preventive effect may be achieved through the many kinds of approaches: Maidong is relatively more enduring than dexamethasone combine cefalexin, and Maidong had less side effects.