Abstract
Introduction: The peritoneal macrophage is a component of the first line of defense against abdominal infections and injury. A majority of work on signal transduction to endotoxin has been carried out on elicited and consequently activated macrophages. Here we examine signaling pathways in resident (unstimulated) macrophages in response to bacterial endotoxins. Methods: Macrophages were isolated aseptically from mice by gentle lavage of the peritoneal cavity with PBS and then purified by attachment to plastic (90-95% phagocytic cells).
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