P27 and MIB-1 Expression Is Related to Malignancy Recurrence in Laryngeal Carcinoma Treated with Partial Laryngectomy: Preliminary Results

Abstract

Considerable evidence has confirmed that p27 protein plays a negative role in cell-cycle progression from the G1 to the S phase and is considered a tumour suppressor. p27 down-regulation was demonstrated in several malignancies. Only a few studies investigated p27's potential prognostic role in laryngeal squamous cell carcinoma (SCC). The aim of the present study was to determine the prognostic relevance of p27 expression in a cohort of laryngeal SCCs that were very homogeneous from a treatment viewpoint to avoid possible influences of treatment modalities on results; all patients underwent only partial laryngectomy on the primary lesion. We simultaneously investigated monoclonal antibody against a proliferating cell associated epitope MIB-1 expression. Twenty-two cases of laryngeal SCC that had undergone exclusive supracricoid or supraglottic laryngectomies with or without neck dissection at the Department of Otolaryngology of Padova University were evaluated. Primary laryngeal SCC p27 reactivity and MIB-1 reactivity were immunohistochemically tested and evaluated by a workstation image analysis system. A lesion was considered p27 positive when more than 18.56% of the tumour cells showed diffuse strong staining. Samples with > 20.24% of stained cells were considered MIB-1 positive. The mean p27 expression was 17% and 22% in patients with and without disease recurrence, respectively. Low p27 expression was statistically associated with disease recurrence (p = .045); on the other hand, high MIB-1 values were associated with SCC recurrence after treatment (p = .045). The intervals of confidence analysis showed a stronger relationship of MIB-1 versus p27 in predicting disease recurrence in our cohort of patients treated only with partial laryngectomies.