P267 In vitro efficacy of inhalative antibiotics against Pseudomonas aeruginosa using artificial sputum medium

Abstract

Cystic fibrosis (CF) is characterized by a polymicrobial infection of the airways with Pseudomonas aeruginosa being the most common pathogen. Chronic infection with P. aeruginosa has been associated with accelerated lung function decline and premature death. In case P. aeruginosa early eradication therapy fails, long-term antibiotic maintenance treatment is required to reduce airway inflammation and irreversible structural lung damage. Most commonly, inhalative antibiotics (tobramycin, colistin, aztreonam lysine, levofloxacin), that generate high local concentrations with low systemic drug concentrations and low systemic side effects, are used. So far, no validated antimicrobial breakpoints to assess antipseudomonal activity of inhalative agents exist. Our study thus aims to investigate the in vitro efficacy of antipseudomonal inhalative antibiotics.