P266 Genomic and phenotypic comparisons of Pseudomonas aeruginosa ST27 strains isolated from respiratory tract and domestic environment of a cystic fibrosis patient

Abstract

Pseudomonas aeruginosa is the major environmental opportunistic pathogen in cystic fibrosis (CF), able to chronically colonize patient’s airways. However, P. aeruginosa acquisition sources are poorly known. A patient attending the Montpellier CF center was sporadically colonized in 2010 by a strain of P. aeruginosa of sequence type (ST) 27 and strains of identical genotype were isolated from all water points in his home during environmental investigations in 2015 and 2020. Our objectives were: i) to compare characteristics of clinical and environmental strains to identify properties that could favor transmission to patient, ii) to study the environmental strains’ evolution between 2015 and 2020 to search for features involved in environmental persistence.