P238 - Functional Toll-like receptor 5 gene variants are not associated with susceptibility to inflammatory bowel disease in the German population

Abstract

s of the 4th Congress of ECCO the European Crohn’s and Colitis Organisation S105 P238 Functional Toll-like receptor 5 gene variants are not associated with susceptibility to inflammatory bowel disease in the German population J. Seiderer1 *, J. Glas1, J. Diegelmann1, B. Sandner1, S. Pfennig1, H. Torok1, B. Goke1, T. Ochsenkuhn1, M. Folwaczny2, B. Muller-Myhsok3, S. Brand1. 1University of Munich Klinikum Groshadern, Munich, Germany, 2Clinic for Preventive Dentistry and Parodontology, LMU Munich, Munich, Germany, 3Department of Human Genetics, Max-Planck-Institute for Psychiatry, Munich-Schwabing, Munich, Germany Introduction: Toll-like receptor 5 (TLR5), the pattern recognition receptor for bacterial flagellin, plays an important role in innate immunity. We recently identified TLR4, the receptor for lipopolysaccharide, as a susceptibility gene for Crohn’s disease (CD) (Inflamm Bowel Dis 2005;11:645 52). We therefore investigated the role of TLR5 variants on susceptibility and phenotype of inflammatory bowel disease (IBD). Aims and Methods: Genomic DNA from 594 Caucasian individuals including 190 patients with CD, 139 patients with ulcerative colitis (UC), and 265 healthy unrelated controls) was genotyped for the SNPs rs5744168=p.Arg392X, rs2072493=p.Asn592Ser, and rs5744174=p.Phe616Leu in the TLR5 gene. Results: None of the three TLR5 SNPs investigated was found to be associated with CD or UC susceptibility. The detailed results of the genotyping analysis are shown in Table 1.