P207 Genetic alterations in nasopharyngeal carcinoma tissues

Abstract

Aim Nasopharyngeal Carcinoma is the most-studied Virus associated cancer. EBV has been shown to induce loss of heterozygosity in chromosome 11. Here we aim to examine if EBV-positive nasopharyngeal carcinoma (NPC) show genetic alterations that impact the tissue identity using STR assay. Methods Fourteen untreated NPC patients were analyzed in this study by comparing 15 STR loci polymorphisms from both nasopharyngeal cancerous tissues and blood samples taken from the same individual. After DNA extraction, MSI and tissue identity testing targeting 15 STR loci were performed using AmpFlSTR® Identifiler® Plus PCR Amplification Kit and analyzed using Genetic Analyzer 3130. Results The data show that genetic alterations (MSI and/or LOH) were observed in 11/14 (78.5%) of NPC patient tissues. MSI was observed in 6 samples (42.8%) and LOH was observed in 7 samples (50.00%), while both LOH and MSI were detectable in 3 samples (21.4%). MSI alone was observed in five markers (32.5%): D51S11, CSF1P0, D19S433, TP0X and D18S51 whereas LOH was observed in 32.5% of markers: D7S820 (7q21.11), D3S1358 (3p21.31), TH0l (11p15.5), D16S539 (16q24.1), D2S1338 (2q35) respectively. Both MSI and LOH were observed in vWA (6.25%). Neither MSI nor LOH was detected in D8S1179, D13S317, 5S818, FGA and Amelogenin markers. Conclusions In this study, we show for the first time that nasopharyngeal carcinoma (NPC) show multiple genetic alterations in the tissue identity and open the door for further investigations in understanding the relation between these alterations and NPC diagnostics and prognostics.