Abstract
Methods A total of 42 subjects (8 acutely and 34 recently HIVinfected individuals with estimated time of seroconversion) were included. Gag quasispecies were analyzed by single-genome amplification/sequencing. Ten amino acid positions across Gag with the most frequent mutations were chosen for analysis. Mutations were analyzed at frequent time points (median 4.5 per subject; IRQ 3.0; 6.0). High-resolution typing was performed within HLA-A, -B, and -C loci. Gag mutations toward the wild type were treated as reversions and mutations from the wild type as escapes. Mutations were classified as minor, transient, dominant, or complete.
Highlights
Open AccessAddress: 1Immunology & Infectious Diseases, Harvard School of Public Health, Boston, MA, USA and 2Botswana-Harvard Partnership, Gaborone, Botswana
Questions addressed in this study included: ''What Gag mutations are most frequent in primary HIV-1C infection?'', ''When do these mutations occur in relation to the estimated time of seroconversion?'', and ''How are these mutations associated with MHC class I HLA alleles?''
Distribution of Gag mutations revealed the dominance of the most frequent Gag mutations within p2/p7/p1/p6, which was in line with the viral diversity within Gag cleavage products
Summary
Address: 1Immunology & Infectious Diseases, Harvard School of Public Health, Boston, MA, USA and 2Botswana-Harvard Partnership, Gaborone, Botswana. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):P382 doi:10.1186/1742-4690-6-S3-P382. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
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