P198 Low-grade inflammatory response to overfeeding is linked to the relative variations of sCD14 and LBP in healthy men

Abstract

Introduction Low-grade inflammation is now recognized as a hallmark of obesity pathophysiology. Recent interest has focused on the role of endotoxins absorbed during high fat meals. LBP and sCD14 have also been suggested as clinical markers of endotoxemia and in mice, the ratio LBP/sCD14 has been associated with high-fat diet induced inflammation. We tested the hypothesis that healthy subjects develop inflammation differently during overfeeding-induced weight gain according to the changes of LBP/sCD14 ratio in plasma. Patients et methodes Eighteen lean to overweight subjects were submitted to overfeeding (OF) during 8 weeks (+ 760 kcal/day). Endotoxemia, sCD14, LBP and IL-6 were measured before and after OF at fasting (n = 18), and in the postprandial phase after an 882-kcal test meal in a subcohort (n = 8). Resultats In the cohort, no effect of OF was observed on fasting IL-6 but OF induced an increase of LBP/sCD14 ratio (P = 0,017). Subjects were divided into tertiles regarding their variation of LBP/sCD14 ratio due to OF. Subjects in the highest tertile (+ 90 % of LBP/sCD14 ratio) increased plasma IL-6 (+ 26%) compared with the lowest tertile (no increase of LBP/sCD14 ratio ; − 31 % of plasma IL-6 ; P Conclusion Overfeeding increases postprandial endotoxemia but the inflammatory outcome appears to be modulated by the handling of endotoxins by different transporters in plasma. This study supports a new concept whereby the setup of low-grade inflammation during the initial phase of weight gain is linked to the relative variations of LBP and sCD14.