P18 High-sensitivity and rapid UPLC-MSn assay effective components in Radix salviae miltiorrhizae in rat brain microdialysate

Abstract

Radix salviae miltiorrhizae , a well-known traditional Chinese medicine (TCM), is the dried root of Salvia miltiorrhiza Bunge . It has been widely utilized in many TCM prescriptions for treating various cardiovascular diseases and cerebrovascular diseases. In comparison with the traditional method using whole brain tissue homogenate, in situ microdialysis is a powerful tool for continuous sampling and analysis of neurochemical substances or drugs in the extracellular fluid of the brain, which can provide advanced information on metabolic pathways in a very small sample volume. The present report describes a highly sensitive and rapid UPLC-MS n method for simultaneous determination of seven components in Radix Salviae Miltiorrhizae using the in situ rat brain microdialysis technique. The reaction selected ions were 197.2/135.1, 137.3/108.3, 359.3/197.3, 493.0/295.2, 718.0/520.0, 297.4/254.3 and 295.5/249.3 m/z for tanshinol, protocatechuic aldehyde, rosmarinic acid, Salvianolic acid A, Salvianolic acid B, cryptotanshinone and tanshinone IIA, respectively. The calibration curves in the range of 2–100 ng·mL −1 for tanshinol, 0.5–100 ng·mL −1 for protocatechuic aldehyde and rosmarinic acid, 1–100 ng·mL −1 for Salvianolic acid A, 2–100 ng·mL −1 for Salvianolic acid B, 0.15–100 ng·mL −1 for cryptotanshinone and 0.5–100 ng·mL −1 for Tanshinone IIA were all with good linearity. The analysis method is sensitive, rapid and suitable enough to be applied in the pharmacokinetic study of the seven main effective components in Radix Salviae Miltiorrhizae . Microdialysis combined with the UPLC-MS n method may provide greater depth to further studies of their pharmacokinetic profiles in rat brain.