P17.61 * ANTI-TUMOR EFFECTS OF S14161 IN GLIOBLASTOMA CELLS

Abstract

BACKGROUND: Glioblastoma is the most common and aggressive form of malignant glioma and is very difficult to treat. Controlling glioblastoma cell invasion and angiogenesis is essential to improve the prognosis of glioblastoma patients. It has been reported that the phosphoinositide 3-kinase (PI3K) inhibitor exhibits potent antiumor effects to malignant tumors. The small chemical compound 8-ethoxy-2-(4-fluorophenyl)-3-nitro-2H-chromene (S14161) was recently identified as an inhibitor of PI3K. Our study aimed to evaluate the antitumor effects of S14167 on two human glioblastoma cell lines (U87MG, T98G) and the molecular mechanisms underlying these effects. METHODS: The anti-invasive effect of S14161 was analysed by an in vitro invasion assay. An in vitro angiogenesis assay was also performed. In vitro growth inhibition of glioblastoma cells by S14161 was determined by the WST-1 assay. The anti-angiogenic effect of S14161 was analysed by an in vitro angiogenesis assay. RESULTS: S14161 suppressed the proliferation of glioblastoma cells. S14161 also suppressed both the invasion of glioblastoma cells and tumor-induced angiogenesis. Molecular-based studies demonstrated that S14161 suppressed gene and protein expression of angiogenic factors. CONCLUSIONS: The results of the present study suggest that S14161 may be a new therapeutic agent for glioblastomas.