Abstract
Introduction Axl receptor tyrosine kinase (Axl) has been known to play a crucial role in cell survival, proliferation, and migration, but little is known about its regulatory role in the migration of gd T cells. Here we investigated the role of Axl in the migration of intraepithelial gd T cells. Methods Primary intraepithelial lymphocytes (IELs) and gdT cells were isolated from 6 to 8 weeks old WT and Axl-deficient mice. Gene expression of IL-7Ra, CCR5, CCR9 and integrin aEb7 was examined by realtime-PCR. IEL phenotype was analyzed by flow cytometry. For adoptive transfer, primary IELs isolated from donors were injected into orbital vein of recipient mice after 6 weeks. Results The gd T cell population and absolute cell number was increased in IELs of Axl-deficient mice compared to them of wild type mice but not in other tissues. In addition, up-regulation of IL-7Ra, CCR5, CCR9 and integrin aEbexpression was observed in both IELs and gd T cells purified from IELs of Axl-deficient mice. The adoptive transfer of IELs of Axl-deficientmice to RAG-deficientmice induced the increased migration of gdT cells. Conclusion Our findings suggest that Axl is required for the migration of intestinal gd T cells by the transcriptional regulation of IL-7Ra, CCR5, CCR9 and integrin aEb7.
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