Abstract
Methods To detect novel subdominant determinants in HIV-1 Gag, we primed CD8+ T cells from eight seronegative donors with autologous dendritic cells transduced to express Gag. T cells were re-stimulated weekly with monocytes pulsed with 123 15-mer overlapping peptides (OLPs) spanning Gag. Responses were identified with OLP matrix pools followed by interrogation at the single OLP level in responsive pools using IFN-γ ELISPOT assays.
Highlights
Recapitulating immunodominant A2-restricted HIV-specific CTLs that do not suppress virus in vivo is unlikely to be an effective vaccine strategy
AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
T cells were re-stimulated weekly with monocytes pulsed with 123 15-mer overlapping peptides (OLPs) spanning Gag
Summary
Address: 1Biological Sciences, University of Texas at El Paso, El Paso, TX, USA, 2La Jolla Institute of Allergy and Infectious Disease, La Jolla, CA, USA and 3University of Pittsburg School of Public Health, Pittsburgh, PA, USA. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):P306 doi:10.1186/1742-4690-6-S3-P306. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
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