P-172: Successful clinical pregnancies of cryopreserved human oocytes after vitrification

Abstract

ObjectiveTo assess the feasibility of the vitrification in cryopreservation of human oocytes for clinical application.DesignDescriptive case series.Materials and methodsThe spare oocytes from stimulated cycles were vitrificated with cryoloop, with 40% ethylene glycol, 30% Ficoll 70,and 0.65M Sucrose. While thawing, the base of the solution was PBS containing 10% EG and sucrose of different concentration from 0.8M to 0.6M, 0.3M, and finally to 0.15M. The survival oocytes after thawing were inseminated by ICSI. The fertilized oocytes were further cultured in vitro for 48-72 hours. High-quality embryos were transferred to the patients who had informed consent.ResultsOverall 235 oocytes were vitrificated and thawed. The survival and fertilization rates of oocytes were 71.9% and 72.2%, respectively. Forty-one embryos were transferred to 11 patients. Two patients achieved pregnancies. The first woman delivered twin female healthy babies on Dec 15, 2005. Another one is in ongoing normal pregnancy of 16th weeks. The babies and mothers are all healthy.ConclusionThe feasibility and safety of human oocytes vitrification were confirmed by our clinical results. Subsequent studies should be undertaken for further optimizing cryopreservation of human oocytes. ObjectiveTo assess the feasibility of the vitrification in cryopreservation of human oocytes for clinical application. To assess the feasibility of the vitrification in cryopreservation of human oocytes for clinical application. DesignDescriptive case series. Descriptive case series. Materials and methodsThe spare oocytes from stimulated cycles were vitrificated with cryoloop, with 40% ethylene glycol, 30% Ficoll 70,and 0.65M Sucrose. While thawing, the base of the solution was PBS containing 10% EG and sucrose of different concentration from 0.8M to 0.6M, 0.3M, and finally to 0.15M. The survival oocytes after thawing were inseminated by ICSI. The fertilized oocytes were further cultured in vitro for 48-72 hours. High-quality embryos were transferred to the patients who had informed consent. The spare oocytes from stimulated cycles were vitrificated with cryoloop, with 40% ethylene glycol, 30% Ficoll 70,and 0.65M Sucrose. While thawing, the base of the solution was PBS containing 10% EG and sucrose of different concentration from 0.8M to 0.6M, 0.3M, and finally to 0.15M. The survival oocytes after thawing were inseminated by ICSI. The fertilized oocytes were further cultured in vitro for 48-72 hours. High-quality embryos were transferred to the patients who had informed consent. ResultsOverall 235 oocytes were vitrificated and thawed. The survival and fertilization rates of oocytes were 71.9% and 72.2%, respectively. Forty-one embryos were transferred to 11 patients. Two patients achieved pregnancies. The first woman delivered twin female healthy babies on Dec 15, 2005. Another one is in ongoing normal pregnancy of 16th weeks. The babies and mothers are all healthy. Overall 235 oocytes were vitrificated and thawed. The survival and fertilization rates of oocytes were 71.9% and 72.2%, respectively. Forty-one embryos were transferred to 11 patients. Two patients achieved pregnancies. The first woman delivered twin female healthy babies on Dec 15, 2005. Another one is in ongoing normal pregnancy of 16th weeks. The babies and mothers are all healthy. ConclusionThe feasibility and safety of human oocytes vitrification were confirmed by our clinical results. Subsequent studies should be undertaken for further optimizing cryopreservation of human oocytes. The feasibility and safety of human oocytes vitrification were confirmed by our clinical results. Subsequent studies should be undertaken for further optimizing cryopreservation of human oocytes.