P1605Increased irregularity and spectral complexity of the intrinsic pacemaker beat-to-beat variability correlates with increased metabolic syndrome components

Abstract

Abstract Background Metabolic syndrome (MetS) is becoming one of the future potential leading risk factors for heart and cardiovascular disease. MetS relates to a condition associated with at least three metabolic risk factors raising risk for health diseases concomitantly such as diabetes, stroke, hypertension, obesity and dyslipidemia. This can lead to chest pain, heart attack, heart damage and overall higher prevalence of cardiovascular disease, atrial fibrillation and sudden cardiac death. One of the underlying mechanisms of the progressive remodeling in presence of MetS components could be altered automaticity, which would reflect modifications of sinus node activity. These phenomena can be evaluated analyzing the components of heart rate variability (HRV). Purpose Our aim was to examine the modifications of sinus node variability in an isolated heart model of diet-induced obesity and MetS. Methods Male NZW rabbits were randomly assigned to high-fat (HF, n=8), control (HF-C, n=7), high-fat, high-sucrose (HFHS, n=9), and control (HFHS-C, n=9) groups, fed with their respective diets during 18/28 weeks. After euthanasia their hearts were isolated in a Langendorff system. We recorded 10–15 minutes of spontaneous activity. Short RR time series were analyzed, and standard HRV parameters were determined with special interest in the time-course of spectral, time-frequency and non-linear components. One-way ANOVA, Kruskal-Wallis test and bivariate correlations were used for statistical analysis (p<0.05). Results We did find an increase in the complexity and irregularity of intrinsic pacemaker activity as shown by modifications of entropy (ApEn: p=0.011 vs HF, p=0.002 vs HF-C, p=0.019 vs HFHS-C) and the complexity index (CI: p=0.006 vs HF, p=0.047 vs HF-C, p=0.027 vs HFHS-C) in HFHS animals (Figure). Higher dispersion on RR differences distributions was observed in the HFHS group. Time-frequency spectral heterogeneity increased in HFHS group (p=0.002 vs HF, p=0.050 vs HF-X, p=0.027 vs HFHS-C) even though no differences were found in standard time and frequency-domain analyses. High-band and low-band spectral concentration ratios showed decreased organization in HFHS when compared to HF (p=0.002) and controls HFHS-C (p=0.027) and HF-C (p=0.050). Interestingly, animal weight and glucose intolerance were highly correlated with the modifications of intrinsic pacemaker variability. Modifications of intrinsic IHRV. Conclusions Modifications of intrinsic HRV seemed to be reliant on the number of components of MetS present, given that only HFHS group showed significant changes towards an increased complexity and irregularity of intrinsic pacemaker variability. Acknowledgement/Funding This work was partially supported by: GV2015-062, CB16/11/00486.