Abstract
In this study, we report that the human p14 ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140–300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14 ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14 ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14 ARF protein is involved in binding to c-Myc and N-Myc proteins.
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