Abstract
Gut microbiota is shown to be linked with response to steroids in some diseases (bronchial asthma). Sufficient changes of intestinal microbiota composition in inflammatory bowel diseases (IBD) patients were shown by numerous investigators. The aim of the study was to evaluate the possible link between changes in the taxonomic composition of the intestinal microbiota in IBD patients with steroid-dependence and steroid-resistance status. The study included 94 IBD patients, including 18 steroid-dependent and 2 steroid-resistant patients. Total DNA was extracted from stool samples followed by whole genome sequencing (SOLiD 5500W). The abundances of 14 genera: Akkermansia, Subdoligranulum, Faecalibacterium, etc. showed statistically significant difference between the studied groups. The abundance of Subdoligranulum (1.67% ± 2.36%), Faecalibacterium (3.21% ± 5.38%) was decreased in steroid-dependent patients compared to patients without steroid-dependency: Subdoligranulum (4.06% ± 6.27%), Faecalibacterium (4.83% ± 4.41%), p<0.05. In steroid-resistant patients Veillonella (10.04% ± 6.70%) and Bacteroides (59.26% ± 12.56%) prevailed comparing to patients without steroid-resistance: Veillonella (0.76% ± 3.55%), Bacteroides (18.80% ± 18.08%). The abundance of Subdoligranulum was decreased: (0.01% ± 0.001%) vs (3.68% ± 5.83%) in patients without steroid-resistance. Surprisingly the abundance of Eubacterium (0.02% ± 0,01%), Faecalibacterium (0.01% ± 0.014%), Roseburia (0.01% ± 0.01%) was quite low in steroid-resistance patients compared to those without steroid-resistance: Eubacterium (1.19% ± 11.98%), Faecalibacterium (4.62% ± 4.63%), Roseburia (2.56% ± 3.98%). Coprococcus, Ruminococcus genus were not detected in steroid-resistance patients. We suppose that steroid-dependence and steroid-resistance status in IBD patients can be partly linked with the gut microbial composition, particularly, decreased number of SCFA-producing bacteria.
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