P.1.065 Effect of the antidepressant nefazodone on the density of cells expressing mu-opioid receptors in discrete brain areas processing sensory and affective dimensions of pain

Abstract

11 Brazilian Annona species were investigated in regard to ecology, growth forms, geographical distribution, karyology and palynology (2n = 14: A. acutiflora, A. ambotay, A. dioica, A. montana, A. muricata, A. pernambucensis, A. nitida, A. salzmannii; — 2n = 28: A. glabra, A. pygmaea; — 2n = 42: A. coriacea). Growth forms vary from large trees (25 m) to small subshrubs (10 cm); vines occur too. These species are allo-to parapatric and inhabit clearly defined ecological niches (mangrove, lowland-rainforest, savanna and mountain-rainforest). The chorological types of the Pernambuco species are: 1) Wide range rain forest distribution: A. montana; 2) small range rain forest distribution: A. salzmannii; 3) relic-endemic mountain rainforest distribution: A. pernambucensis; 4) wide range mangrove distribution: A. glabra; 5) wide range Cerrado-savanna distribution: A. coriacea and A. dioica. The species belong to 5 different sections which are widely distributed throughout South America. All this reflects the dynamic processes of expansion and restriction of forest and savanna types during the long term vegetation history. The lack of local speciation patterns of Annona in Pernambuco indicates a relatively recent invasion of most of the species. Among the pollen tetrads of A. glabra, A. montana and A. pygmaea differences are found in regard to the structure of the foot-layer and the tectum perforation. The eco-systematic differentiation is accompanied by strong karyological changes concerning the ploidy level (not section specific) and interphase nuclei structure (e.g. specific for sect. Eu-Annona). Diploids are mostly restricted to the rain forest, polyploids to extreme habitats. The nuclei of the two tetraploid species differ remarkably: A. pygmaea with two NOR’s and a few big chromocentres, could eventually be an autopolyploid; A. glabra with 4 NOR’s and many small chromocentres, probaly is a young allopolyploid. Polyploids of humic areas are regarded to be different from those in dry areas (e.g. in Annona) in regard to further speciation. The idea of taxon specific preference for maximum speciation on a certain ploidy level is discussed.