P105 S-propargyl-cysteine (ZYZ-802), a novel water-soluble donor of endogenous hydrogen sulfide, attenuates impairment against cerebral ischemia–reperfusion injury in rats

Abstract

Objective Cerebrovascular diseases can do great harm to human health including high rate of mutilation and high mortality and so on. Our Present study was performed in vivo to verify the hydrogen sulfide donor S-propargyl-cysteine, also named ZYZ-802, protective effect in ischemia–reperfusion rats and explore its probable mechanisms as well. Methods 150 SD rats were randomly divided into 6 groups: sham-operate group; focal cerebral ischemia/reperfusion injury group which were subjected to one hour of ischemia followed by 48 h of reperfusion; drug treatment groups (focal cerebral ischemia/reperfusion injury and treatment with different doses of drugs). The behavioral tests were used to evaluate the damage to central nervous system. 2,3,5-triphenyl tetrazolium chloride (TTC) staining method, PET-CT was used to assess the percentage of brain infarct area. H&E staining method was used to observe the pathologic histological changes of hippocampus CA1 region. Cat walk and Rota-Rod Test was used to detect the behavior function. SOD and GSH activity in brain homogenate was determined. Results Compared with sham-operate group, rats with one hour of ischemia followed by 48 h of reperfusion exhibited severe neural injury as shown in significant increase of neurological scores (P Conclusion SPRC exhibits neuroprotective effects on cerebral ischemia/reperfusion injury in rats.