P103 Effects of fast and slow H2S donors on rat hemodynamic parameters

Abstract

H2S is important in the pathogenesis of cardiovascular diseases including hypertension. H2S donors have the viability to be effective therapeutic agents. We compared effects of fast H2S donor Na2S and slow H2S donors AP39 and AP67 on rat hemodynamic parameters in normal and L-NAME treated rats. Drugs were administered into the right jugular vein (i.v.) as bolus and blood pressure (BP) and pulse waveform (PW) was detected by transducer in the cannulated left carotid artery of an anaesthetized Wistar rat. In experiments without L-NAME, Na2S (10–30 μM/kg), AP39 (250–1000 nM/kg) and AP67 (15–30 μM/kg) similarly, but at different concentrations, influenced PW, decreased and increased BP, heart rate and influenced ± dP/dtmax and a position of dicrotic notch. In the presence of L-NAME (i.v., 25 mg/kg), all H2S donors changed PW, decreased systolic BP, but were less efficient on diastolic BP. Their effects were more pronounced and prolonged in the presence of L-NAME than without it. These results contribute to the understanding of the biological effects of H2S which are important in the exploration of H2S donors as new drugs in the treatment of “H2S related diseases”. This work was supported by VEGA 2/0050/13.