Abstract

Introduction Polybrominated diphenyl ether (PBDE) measurement in hair is an important emerging potential biomarker for possible endocrine-disrupting effects in humans. The aim of this study was to evaluate effective removal of environmentally deposited PBDEs while keeping hair intact for measuring incorporated PBDE levels. Methods Non-pooled hair samples from 10 human were subjected to a no wash (control) and three wash treatments: water, SDS solution, and hexane. After the treatments, hair samples (solid phase) and washes (aqueous phase) were analyzed for eight common PBDE congeners using GC/MS using previously established methods. Results The mean total PBDE values for no wash, water wash solid and aqueous phase, SDS solution wash solid and aqueous phases, and hexane wash solid and aqueous phases were: 20.32±9.65, 21.40±10.47, 0.39±0.59, 19.27±5.94, 1.33±2.15, 16.91±9.14 and 1.92±2.76 ng/mg respectively (p Conclusion Washing hair with water is the most effective method for removing external environmental contamination while keeping the matrix of the hair and PBDE congeners undamaged.

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