Abstract
Methods We used platelets sampled from healthy, HIV-1,2 negative, normal blood donors. Platelet-Rich Plasma (PRP) was incubated after addition thrombin receptor agonist peptide or HIV env glycoprotein. PRP were centrifuged and platelet free supernatant was stored. Two recombinant gp120MN (produced in insect cells using the baculovirus expression system) and synthetic peptides of gp41MN, and mAbs to gp41: D50 and 2F5, were all obtained through the AIDS Research and Reference Reagent Program. Soluble CD62p and RANTES production in cultures were measured by specific ELISA.
Highlights
Open AccessAltered release of RANTES from human, normal platelets: contribution of distinct HIV-1MN gp peptides
Blood platelets link the processes of haemostasis and inflammation
This study examines the secretion of normal platelets after exposure to recombinant HIV-1MN-gp120 or gp41 peptides
Summary
Altered release of RANTES from human, normal platelets: contribution of distinct HIV-1MN gp peptides. F Cognasse*, H Hamzeh-cognasse, J Berthet, P Damien, F Lucht, B Pozzetto and O Garraud. Address: Immunology, EFS Auvergne-Loire – GIMAP-EA3064, Saint-Etienne, France * Corresponding author from AIDS Vaccine 2009 Paris, France. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):P140 doi:10.1186/1742-4690-6-S3-P140. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
