P1-5-19 - Study of the gene expression of esophageal squamous cell carcinoma-associated novel candidate genes

Abstract

Background: In Taiwan, most esophageal cancer was squamous cell carcinoma; mainly happened in men, the incidence rate increased from 5.7/105 in 1995 to 17.7/105 in 2011. The ive-year survival rate was less than 15%. It becomes crucial to identify the potential genes for the prediction of esophageal cancer malignancy. In our previous study, three Taiwanese and one Caucassian ESCC cell lines as well as three normal tissues of esophagus were analyzed by using microarray technique. We have identified the most significant 10 down-regulated (DCN = PRELP, HBB, C7, HBA1, DES, COX7A1, PLVAP, IGHG4 and MYL9) and 13 up-regulated (HMGA2, ECT2, UBAP2L, ZIC2, COPA, IMP-2, HOXC13, WARS, FJX1, HOXA10, HOXD11, ADPGK and IMP-3) novel genes. Methods: In this project, we verify those potential novel genes in the RNA levels. The 23 candidate genes selected from our previous study were then investigated their gene expression using [email protected] Gene Expression Assay. 4 cancer cell line (48T, 81T, 146T, OE21) RNA were compared with a human normal esophgeal cancer RNA mixture which contains 5 asian donor RNA (BioChain). Genes with expression fold changes between samples larger than 2 are considered as differential gene expressions. Result: We found that in our down-regulated genes, DCN, HBB, C7, HBA1, DES, COX7A1, IGHG4 show low gene expresisions in all cell lines.(fold change from -47.25 ∼ -17845.58, p < 0.05); PRELP, PLVAP, MYL9 also show low gene expresisions but no statistically differences. In up-regulated group, no significantly differences were found in CE48T cell line, but HMGA2, ECT2, ZIC2, IMP-2, HOXC13, HOXA10, IMP-3 all show high gene expresisions in other 3 cell lines (fold change from 3.26 ∼ 100.45, p < 0.05). Conclusion: Compared to the findings from cell lines, we found the high consistency in terms of RNA expressions in those candidate genes, allowing us to be more confidence that these candidate genes we selected from our study may play an important role in the occurrence of ESCC.