P1.01 Clinical Application of Plasma MIR-34B-3P and MIR-302A-5P in the Diagnosis of Non-Small Cell Lung Cancer

Abstract

MicroRNA is a kind of single-stranded non-coding RNA whose length is about 22 nucleotides and its abnormal expression is related to disease closely. This study is aiming to explore the relative expression of miR-34b-3p and miR-302a-5p in the plasma of non-small cell lung cancer (NSCLC) patients and its clinical value. The levels of miR-34b-3p and miR-302a-5p in plasma were detected by real-time polymerase chain reaction (RTPCR) in 86 patients with NSCLC, 64 patients with pulmonary tuberculosis (PTB) and 39 healthy subjects. Analyze their value in diagnosing NSCLC by contrasting and combining carcino-embryonic antigen (CEA), neuron- specific enolase (NSE), and cytokeratin 19 fragments 21-1 (CYFRA21-1) The levels of plasma miR-34b-3p and miR-302a-5p in NSCLC group were significantly higher than those in the PTB group and the healthy group (P<0.05). In patients with NSCLC, the levels of plasma miR-34b-3p was correlated with the diameter of tumor (P<0.01). When using one plasma marker to diagnose NSCLC, miR-302a-5p had the highest sensitivity (82.6%) and CEA had the highest specificity (81.6%). While combined two plasma markers, miR-34b-3p+miR-302a-5p had the highest sensitivity (80.2%) and miR-34b-3p+CEA had the highest specificity (81.4%). As detected multiple markers, miR- 302a- 5p+ NSE+ CYFRA21-1 had the highest sensitivity (81.4%) and miR-34b- 3p+CEA+ NSE had the highest specificity (90.3%). The combination of miR-34b-3p, miR-302a-5p and CEA obtained the highest area under the curve (AUC), which was 0.832. Logistic regression model indicated that miR-34b-3p was independent risk factor for NSCLC compared to control groups. The miR-34b-3p and miR-302a-5p in plasma could be hopefully used as biological markers for the diagnosis of NSCLC.