Abstract
Aim The development of type 1 diabetes (T1D) and its chronic complications may have a genetic background. We aimed to describe HLA haplotypes in patients with T1D from different urban geographical regions of Brazil. Methods This was a cross-sectional, nationwide survey conducted in 14 public clinics from 10 Brazilian cities. HLA Class II Typing were performed with medium to high-resolution PCR-RSSO (LabType SSO, One lambda Inc.) in 561 T1D patients. Common and Well Documented (version 2.0) was used to defined alleles, and ambiguities were solved by Next Generation Sequencing (NGS) (Holotype HLA Assay, Omixon Inc.). HLA haplotypes were determined in Arlequin v.3.5 software. Results We have determined 125 haplotypes from T1D patients. Top 10 haplotypes are shown in Table 1. Haplotypes from South Brazil T1D patients were significantly different when compared to Southeast and North (p Conclusions Our study showed that T1D patients presented new haplotypes which could be associated with T1D. Further characterization of genomic ancestry, as well as extended haplotypes analysis, should be able to offer a more definitive risk for unfavorable outcome of Brazilian T1D individuals. Download : Download high-res image (443KB) Download : Download full-size image L.M. Porto: 1. Grant/Research Support; Company/Organization; Faperj, CNPq and Omixon.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
