P0833 GASTRITIS CAUSED BY HELICOBACTER HEILMANNII PROBABLY TRANSMITTED FROM DOG TO CHILD

Abstract

Introduction: Helicobacter Helmannii infection rarely affects children but is frequent in dogs. H Helmannii could be a zoonis but proof is lacking. We describe a child affected by his dogs and suggest an animal transmission to child Methods: A12 year old child presented with epigastric burns for 6 months. Endoscopic examination revealed an hyperaemic gastric mucosa. Histologic examination found an acute infiltration of chorionic tissue and H Helmannii like microorganisms. Bacterial culture was negative. Disparition of H Hemanni at endoscopic control was noted after tritherapy treatment. The child was living with 2 dogs. Both were endoscoped. A nodular gastritis was found at endoscopic examination of the brown dog Histopathological examination revealed a follicular gastritis enclosing H Helmannii. The endoscopic examination of the black and white dog found a normal looking gastric mucosa but histological examination found H Helmannii. A molecular analysis was performed in order to compare the bacteria present in the stomach of the child and his two dogs. The only sample available for the child was a gastric biopsy obtained after antibiotic therapy for Helicobacter eradication. After DNA extraction universal primers were used to amplify a fragment of approximately 1,500 bp of 16S rRNA gene. Results: For one dog the sequencing of 400 bp on both ends led to the identification of H. heilmannii. For the second dog an additional nested PCR was necessary and the sequencing of 1100 bp out of 1165 bp led to the identification of H. heilmannii-like organism. Unfortunately no sequence could be amplified from the biopsy of the child Conclusion: Also, using a 23S rRNA PCR, the amplicons obtained from the 3 cases had enough sequence homology to conclude that the same Helicobacter species was probably present.