P078 The relationship between MiHAs compatibility and HLA matching degree of related donor and recipient pairs in allogeneic hematopoietic stem cell transplantation

Abstract

Aim This study is aimed at investigating the relationship between minor histocompatibility antigens (MiHAs) compatibility and human leucocyte antigens (HLA) matching degree of related donor and recipient pairs in allogeneic hematopoietic stem cell transplantation (allo–HSCT). Methods The 1116 samples is consisted of 439 leukemia patients and 677 related potential donors including 229 HLA-matched, 245 HLA-semi-matched and 204 HLA-unmatched groups. PCR-SSP technique was used to genotype the 14 autosomal MiHAs coding genes. Typing data were analyzed for allelic, genotypic and phenotypic frequencies of the 14 MiHAs. The disparity rate of MiHAs in HLA-matched, HLA-semi-matched and HLA-unmatched groups were analyzed respectively. Linkage analysis was performed on PECAM-1A (codon 125) – PECAM-1A (codon 563)-PECAM-1A (codon 670) haplotypes. Results The disparity rates of HA-1,HA-2,HA-8,ACC-1, PECAM-1 (codon 125) V,PECAM-1 (codon 563) N,PECAM-1 (codon 670) G,CD62 (codon 206) and CD62 (codon 213) were observed significantly lower in HLA-matched group than those in HLA-semi-matched group ( P P P P P ≅ 1). Conclusion This is the first time to report the positive correlation between MiHAs matching degree and HLA compatibility of donor-recipient pairs, which provided important data to select best suitable donor for a recipient in allo-HSCT. Keywords: allo-HSCT; MiHAs; HLA; GVHD.