Abstract
Results Cell-free HIV-1 RNA was frequently detected in CVS from patients viremic for HIV RNA in plasma but was unusual in aviremic patients (75% versus 16%, and mean = 5921 copies/ml versus 2696 copies/ml, respectively, P < 0.001). Levels of HIV-1 RNA were positively correlated in CVS and plasma (ρ = 0.7, P < 0.001). CVS contains low T lymphocytes quantities (mean = 120 CD4+ cells/ml and 133 CD8+ cells/ml) and CVS-derived CD4+ T cells are mostly memory and activated lymphocytes (CD45RA-, HLA-DR+, CD38+, CD69+). Those cells were strikingly different from blood CD4+ T cells with a phenotype exhibiting a mucosal profile with higher expression of CD103 combined with lower expression of CCR7. Cell-associated HIV-1 RNA was detectable in only 3/51 CVS including 2 from viremic patients, whereas 28/51 plasma cell-culture supernatants were positive. Levels of cell-associated HIV-1 RNA were higher in blood samples of viremic individuals than in undetectable subjects (P = 0.01).
Highlights
Open AccessImpact of highly active antiretroviral therapy on cell-free and cell-associated HIV-1 in cervicovaginal secretions and blood
Heterosexual contact is a major route for HIV-1 transmission and cervicovaginal secretions (CVS) contain both cell-associated and cell-free virus
Cell-free HIV-1 RNA was frequently detected in CVS from patients viremic for HIV RNA in plasma but was unusual in aviremic patients (75% versus 16%, and mean = 5921 copies/ml versus 2696 copies/ml, respectively, P < 0.001)
Summary
Impact of highly active antiretroviral therapy on cell-free and cell-associated HIV-1 in cervicovaginal secretions and blood. Address: 1Montpellier 1 University, Montpellier, France and 2Centre Muraz, Bobo-Dioulasso, Burkina Faso * Corresponding author from AIDS Vaccine 2009 Paris, France. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):P397 doi:10.1186/1742-4690-6-S3-P397. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts - A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
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