Abstract
Methods We have studied the effects of human milk (HM) and seminal plasma (SP) on interfering with the capture of HIV-1 through binding to DC-SIGN on dendritic cells and prevent the subsequent transfer of HIV-1 to CD4 cells. HM and SP were tested in Raji-DC-SIGN and immature dendritic cell transfer assays to determine interference with HIV-1 capture and transfer. Fractionation and MALDI-TOF biochemical analysis was utilized to identify proteins with DC-SIGN binding activity.
Highlights
An effective HIV-1 vaccine will have to interfere with viral transmission across a mucosal surface and prevent the establishment of infection
We have studied the effects of human milk (HM) and seminal plasma (SP) on interfering with the capture of HIV-1 through binding to DC-SIGN on dendritic cells and prevent the subsequent transfer of HIV-1 to CD4 cells
We identify that bile-salt stimulated lipase (BSSL) and mucin6 have the capacity to bind DC-SIGN and prevent HIV-1 capture at mucosal surfaces
Summary
Natural compounds in bodily fluids which bind DC-SIGN and prevent HIV-1 capture and transfer to CD4 cells. MJ Stax, G Pollakis, RR Sprenger, M Melchers, T van Montfort, RW Sanders, D Speijer and WA Paxton*2. Address: 1Department of Medical Biochemistry, Amsterdam, Netherlands and 2Laboratory of Experimental Virology, Academic Medical Center, Amsterdam, Netherlands. Published: 22 October 2009 Retrovirology 2009, 6(Suppl 3):P108 doi:10.1186/1742-4690-6-S3-P108. AIDS Vaccine 2009 Anna Laura Ross Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
