P069 HOTAIR, A Long Non-Coding RNA, Is a Marker of Abnormal Cell Cycle Regulation in Lung Cancer

Abstract

The dis-regulation of cell cycle is an obvious marker of tumors including lung cancer. Recently, the study of cell cycle inhibitor has made great progress in lung cancer. While a question, what kinds of patients could use cell cycle inhibitor, has plagued us. So seeking an accurate and convenient marker for the abnormal cell cycle in lung cancer is very important. MTT, EdU, Transwell, ChIRP, Western-blot, luciferase reporter assay, Orthotopic nude mouse model, Immunohistochemistry. In the present research we had showed lnc-RNA HOTAIR is an optimal indicator of cell cycle dis-regulation in lung cancer. HOTAIR specific expression in lung primary tumor sample by the analysis of TCGA public database and 67 pairs of patients’ tissues from our department. And through the TCGA public database KEGG analysis, HOTAIR is correlated with cell cycle pathway. Then we identified that HOTAIR and its 2 segments, HOTAIR3’ and HOTAIR5’, promote the cell cycle passing through the restriction point during G1-S phase by regulating Rb-E2Fs pathway and influence NSCLC cell proliferation, migration, and invasion through epithelial-mesenchymal transition (EMT) and β-catenin pathway in vitro and vivo. Last we had also showed the high expression of HOTAIR associated with fast resistance to gefitinb through dis-regulated cell cycle. In conclusion, HOTAIR could be an ideal indicator of cell cycle dysregulation and guide the use of cell cycle inhibitor.