P0660 / #2106: COMPATIBILITY BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF VANCOMYCIN HYDROCHLORIDE AND SODIUM HEPARIN USED IN CENTRAL LINES ANTIMICROBIAL LOCK THERAPY

Abstract

Aims & Objectives: To verify compatibility of vancomycin hydrochloride in association with sodium heparin, according to the influence of time and temperature. Methods: Experimental study, simulating the clinical practice of antimicrobial lock in central intravenous lines performed in severely ill children. The concentration was analyzed in triplicate by High Performance Liquid Chromatography (HPLC). The analytical method was validated. Drugs concentrations were 5mg/mL of vancomycin hydrochloride and 100U.I./mL of sodium heparin in saline solutions. The sample consisted of 06 solutions (03 at 22°C and 03 at 37°C) maintained in the absence of light. The HPLC analyzes occurred at times zero (T0), 3 (T3), 8 (T8) and 24 (T24) hours resulting in 72 chromatograms. Results: The vancomycin hydrochloride and sodium heparin solutions at 22°C resulted in a reduction (p < 0.001) of vancomycin hydrochloride concentration in 24 hours (4.82±0.08mg/mL; 96.34±1.70%); the same solution at 37°C resulted in vancomycin hydrochloride lower concentrations (p=0.001) since the initial moment of drugs association (T0=4.75±0.09mg/mL or 94.91±1.76%; T3=4.76±0.06mg/mL or 95.12±1.29%; T8=4.75±0.16mg/mL or 95.05±3.15%; T24=4.85±0.06mg/mL or 96.94±1.23%). During drugs association, at both temperatures, precipitate formation was observed after three hours. Conclusions: The concentration of vancomycin hydrochloride associated to sodium heparin presented variations below 10% leading to the conclusion of chemical compatibility until 24 hours. The solutions showed physical incompatibility after 3 hours of drugs association.