P029 : HLA-A, -B, -DRB1 ALLELE AND HAPLOTYPE FREQUENCIES IN RENAL TRANSPLANT CANDIDATES IN A POPULATION IN SOUTHERN BRAZIL

Abstract

To evaluate the diversity of alleles and haplotypes of HLA-A, -B and -DRB1 in renal transplant candidates in a population in southern Brazil. The frequencies of the HLA-A, -B and -DRB1 alleles and haplotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Paraná State, southern Brazil. Patients were classified according to ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans and Amerindians; browns or “pardos”] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence-specific oligonucleotide method (PCR-SSO), combined with Luminex technology. In the analysis of the total samples, 20 HLA-A, 32 HLA-B and 13 HLA-DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA-A ∗ 02 (25.4%), HLA-B ∗ 44 (10.9%) and HLA-DRB1 ∗ 13 (13.9%). The most frequent haplotypes were HLA-A ∗ 01-B ∗ 08-DRB1 ∗ 03 (2.3%), A ∗ 02-B ∗ 44-DRB1 ∗ 07 (1.2%) and A ∗ 03-B ∗ 07-DRB1 ∗ 11 (1.0%). Significant differences ( p < 0.05) were observed in the HLA-A ∗ 68, B ∗ 08 and B ∗ 58 allele frequencies among ethnic groups. Data from this study provide knowledge of the frequencies of the HLA-A, -B and -DRB1 allele and haplotypes in renal transplant candidates from north/northwestern Paraná state, southern Brazil.