P-026 Frequency of thrombophilia in females with stroke in association with oral contraceptive use

Abstract

s / Thrombosis Research 131, Suppl. 1 (2013) S71–S103 S83 P-025 Adrenomedullin is associated with vonWillebrand factor activity in non-pregnant but not in pregnant women M. Poyhonen-Alho1, R. Lassila 2, L. Joutsi-Korhonen2, G. Nicholls 3, R. Kaaja 4 1Department of Obstetrics and Gynaecology, Helsinki University Central Hospital/Jorvi Hospital, Helsinki, Finland; 2Coagulation Unit, Departments of Hematology and Clinical Chemistry (HUSLAB Laboratory Services), Helsinki University Central Hospital, Helsinki, Finland; 3Christchurch Hospital, University of Otago, Christchurch, New Zealand; 4Turku University and Satakunta Central Hospital, Pori, Finland Introduction: Adrenomedullin has many cardiovascular beneficial effects, such as vasodilation, angiogenesis and tolerance of cells to oxidative stress and hypoxic injury. In addition, adrenomedullin has shown to augment the release and production of tissue factor pathway inhibitor in human aortic endothelial cells suggesting a role in anticoagulation on endothelial surface. Objective: We aimed to study the nocturnal variability of coagulation and plasma adrenomedullin in gestational diabetes, normal pregnancy and in non-pregnant women. Methods:We studied 41 women with gestational diabetes, 22 healthy pregnant and 14 non-pregnant controls. We assayed serial nocturnal changes of plasma adrenomedullin and coagulation variables, including factor VIII activity (FVIII:C), von Willebrand factor ristocetin cofactor activity (vWF:RCo), antigen (vWF:Ag) and collagen binding activity (vWF:CB) at 12 pm, 4 am and 7 am. Results: Adrenomedullin was higher in pregnant compared to non-pregnant women with no difference between the pregnant groups. In gestational diabetes, FVIII:C was lower than in normal pregnancy. AM correlated strongly with vWF:Ag (ρ=0.83, P=0.000), vWF:RCo (ρ=0.80, P=0.001) and vWF:CB (ρ=0.68, P=0.010) in non-pregnant controls. In contrast, these correlations disappeared in both pregnant groups. No correlation of adrenomedullin to FVIII:C existed within the study groups. Conclusions: These findings strengthen the view of adrenomedullin as a relevant factor in human coagulation. Distinct correlations of adrenomedullin with vWF and FVIII, usually working in tandem, could suggest an unravelled mechanism which is involved in the coupling of vWF with FVIII. This finding needs more insight from future studies. P-026 Frequency of thrombophilia in females with stroke in association with oral contraceptive use P. Dulicek1, M. Kost’a 1, P. Sadilek1, M. Beranek2 1IVth Department of Internal – Hematology; 2Department of Clinical Biochemistry, University Hospital and Medical Faculty Hradec Kralove, Czech