P022 Therapeutic effect of adipose tissue-derived stem cell in Interleukin-10 knockout mice

Abstract

Background: Inflammatory Bowel Disease (IBD) is characterized by chronic intestinal inflammation with unknown etiology. Interleukin-10 Knockout (IL-10 KO) Mice are known to develop intestinal inflammation that resemble CD. We investigated if a human adipose-derived stem cells (h-ASCs) are capable to attenuated colon inflammation in IL-10 KO mice. Methods: h-ASCs were isolated from adipose tissues obtained from human donors. Colitis was induced in the mice by administration of piroxicam orally. Mice treated with intraperitoneal h-ASCs injection (5×105 cells) or normal saline as control after induction of colitis. All mice were euthanized 14 days after colitis. Histopathologic score and level of inflammation related cytokines were evaluated. Comparisons were made between h-ASCs treated or control non-treated IL-10 KO mice and wild type littermate. Results: The flow cytometry showed higher expression of CD105, CD73, CD90, CD44, however, hematopoietic stem cell markers such as CD45, CD34 and CD31 were not expressed in ADSC. Clinical score was lower in h-ASCs treated IL-10 KO mice than in control. Histopathologic assesment of large intestine showed lower inflammatory cells infiltration and less mucosal damage in h-ASCs treated mice than in control. Tissue score was lower in h-ASCs treated IL-10 KO mice than in control. The level of Monocyte chemoattractant protein-1 and Toll-like receptor 4 expression in the large intestine was lower and anti human IL-10 and anti mouse IL-4 in the serum was higher in h-ASCs treated mice. Conclusions: These findings suggested that administration of h-ASCs show beneficial effect on T-cell induced spontaneous colitis model and emerge a therapeutic option in inflammatory bowel disease.