P-0205 Activity of Antioxidant Enzymes in Colorectal Cancer

Abstract

ABSTRACT Introduction The development of colorectal cancer is a multistage process associated with multiple somatic mutations and abnormalities in cell proliferation. One of possible factors related to these disorders is the oxidative stress associated with increased production and levels of reactive oxygen species and / or activity inhibition of antioxidant barrier in cancer cells. The aim of this study was to examine selected parameters of antioxidant barrier in colorectal cancer. The activity of superoxide dismutase, glutathione peroxidase and malondialdehyde, which is the product of lipid peroxidation was determined. Methods Enzyme activity was studied in normal and tumor tissues taken from patients with colorectal cancer. The study group included 72 patients: 13 with a cancerous tumor located in the right half of the colon, 16 with the left half of colon tumor, 18 of the sigmoid colon cancer and 25 with rectal cancer. Samples of tumor tissue and healthy mucosa were collected from each patient. Enzyme activity was determined according to the location of the tumor, clinical stage (S) and histopathological grading (G). The testing ready-to-use kits were used for the determination of Oxis Research International, Inc. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH) and malondialdehyde (MDA) was determined. Results A statistically significant increase of activity of superoxide dismutase (SOD), glutathione peroxidase (GSH) and malondialdehyde (MDA) was found in tumor tissue compared with normal tissue. There were no significant differences in the activity of the enzymes depending on the location of the cancerous tumor, clinical stage of cancer or histopathological grading (G). Conclusion 1. Increased antioxidant potential in colorectal cancer may be associated with increased levels of reactive oxygen species and an increase in generation of lipid peroxidation products. 2. Clinical stage and histopathological grading of the tumor does not alter the activity of antioxidant enzymes. 3. The activity of antioxidant enzymes does not change depending on the location of the cancerous tumor.