P0161TO EVALUATE THE TH PROFILE (TH1/TH2/TH17 ) AMONG GESTATIONAL DIABETES AND NORMAL PREGNANCY

Abstract

Abstract Background and Aims Gestational diabetes mellitus (GDM) is a common pregnancy complication, characterized by insulin resistance and low-grade systemic inflammation with a pro-inflammatory immune system response. Our objective was to study the peripheral Th1, Th2, Th17 and Treg response in GDM compared to normal pregnancy. Method Th1, Th2, Th17 and Treg subsets was determined by flow cytometry based on staining for specific intracellular cytokines levels and their CRP levels were also assessed.. The health status of all offspring was also assessed 6 months post-delivery. Results Α total of 32 adult pregnant women were enrolled into a GDM (n=18) and Control (n=14) group. At the third trimester of pregnancy, the GDM group had a higher proportion of Th2, Th17 and Treg cells compared to control. Contrary to the control group, the GDM group exhibited no significant change in the Th1/Th2/Th17/Treg profile postpartum. A higher circulating CRP were noted in the GDM group compared to controls. At the 6-month post-delivery assessment, 23.6% of the offspring from the GDM group were found to have developed atopic dermatitis or food allergy compared to none from the control group. Conclusion Compared to an uncomplicated pregnancy, GDM exhibits a significantly different peripheral T-cell profile at the third pregnancy trimester characterized by higher proportion of Th2, Th17 and Treg cells which persist six months post-delivery, while the increased high sensitivity CRP (hsCRP) levels stressed the low-grade inflammatory profile of this disease