P0105 Flavonoid fraction of Citrus reticulata juice inhibits both proliferation and migration of anaplastic thyroid carcinoma cells

Abstract

Background Natural extracts from various citrus species have shown promising antitumour effects. In this study we evaluated the effects of the flavonoid fraction of Citrus reticulata (mandarin) juice (FFMJ) on both proliferation and migration of three human anaplastic thyroid carcinoma (ATC) cell lines. Methods FFMJ was analysed by uHPLC to elucidate its components. Findings Treatment of CAL-62, C-643, and 8505C cells with rising concentrations of FFMJ significantly reduced cell proliferation in a concentration-dependent and time-dependent manner, with maximal effect elicited at 0.5 mg/ml concentration after 48 h of incubation. Moreover, cytofluorimetric analysis showed a block of the cell cycle in the G2/M phase, accompanied by low cell mortality resulting from autophagic death. Furthermore, FFMJ inhibited migration of ATC cells and was associated with decreased activity of the metalloproteinase MMP-2. Interpretation These finding demonstrate that FFMJ exerts antiproliferative effects on ATC cells together with reducing their migration properties, suggesting a promising role in the prevention or treatment of thyroid cancer.