P0093 MicroRNA-497 inhibits the invasion and metastasis of colorectal cancer by targeting vascular endothelial growth factor-A

Abstract

Background MicroRNAs (miRNAs), which are non-coding RNAs that regulate gene expression, are involved in tumour metastasis. In this study, we describe the downregulation and function of miR-497 in colorectal cancer (CRC) metastasis. Methods We compared expression of miR-497 in CRC tissues and corresponding tissues without tumours. Findings MiR-497 was underexpressed in 34 CRC tissues compared with their corresponding tissues without tumours and in the HCT8, LOVO, Ls-174, HCT116, and HT29 cell lines. Overexpression of miR-497 did not inhibit CRC cell growth but suppressed CRC cell metastasis and invasion in vitro and in vivo. MiR-497 might suppress invasion and metastasis of CRC cells by targeting VEGF-A. We also found that overexpression of miR-497 altered the expression of key components in the VEGF-A/ERK/MMP-9 signalling pathway. Interpretation miR-497 can function as an inhibitory factor in the metastasis of CRC, demonstrating the potential of this molecule as a promising novel therapeutic target. This study also revealed that the miR-497/VEGF-A/ERK/MMP-9 signalling pathway is involved in the metastasis of CRC.