Abstract
A p-HEMA (poly-hydroxy ethyl methacrylic acid) membrane using alcohol oxidase deposited on a carbon screen printed electrode (SPE) as working electrode for amperometric transducer has been built for biochemical sensor. Acetophenon derivate has been applied to generate polymeric cross linking while ferrocene mediator has been added to wire the electrochemical reaction. The UV photocuring attached the membrane polymer onto the carbon SPE. This study has obtained that nanogold application enhanced the formaldehyde biosensor performance. Optimum result using cyclic voltametric (CV) mode in amperometric assay has been found by applying 1.6% acetophenon, 1% ferrocene, 7.7u alcohol oxidase and 0.2% nanogold in phosphate buffer of pH 7.2 at 500s UV photocure.
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