Abstract
There has been increasing interest in the use of DNA fragmentation as a marker of male infertility. Recently, it has been recognized that the use of sperm with a high percentage of DNA damage results in a lower pregnancy rate with IVF and a lower incidence of blastocyst development after ICSI. Increased DNA damage has also been linked to recurrent miscarriages. Sperm DNA integrity is usually evaluated by either flow cytometry (DNA Integrity Assay) or a slide based technique [TUNEL (terminal deoxynucleotidyl transferase - mediated deoxyuridine triphosphate nick end labeling)].
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