P-717 Segmental aneuploidies are not related to paternal age in young women

Abstract

Abstract Study question Is there an impact of paternal age on embryo segmental aneuploidy rate? Summary answer Even when segmental aneuploidies are most linked to paternal origin, results do not show a relationship between paternal age and embryo segmental aneuploidies. What is known already Most studies consider maternal age as a risk factor for spontaneous abortion, infertility and genetic defects in the offspring. However, attention has only recently turned to the impact of paternal age on reproductive outcome. In Assisted Reproduction Technology male age has been related to a decrease in sperm quality and clinical outcomes and an increase in sperm DNA damage has been identified among elderly males. Studies assessing the type of chromosomal aneuploidy associated with paternal aging have reported mixed relative risk results for chromosomes 13, 18, 21, and X. Segmental aneuploidies are most linked to paternal origin. Study design, size, duration Observational, retrospective and multicentric study. The study was approved by the local institutional review board (IRB). All the patients underwent an IVF cycle with oocytes from egg donors, followed by Preimplantation Genetic Testing for Aneuploidy (PGT-A) at IVI-RMA Madrid, IVI-RMA Valencia and IVI-RMA Barcelona from January 2018 to August 2022. A total of 3412 biopsied embryos obtained from 660 cycles were included in the study. Participants/materials, setting, methods Exclusion criteria were the following: altered karyotype, single gene disorder and male factor (sperm concentration lower than 5 million/ ml). Embryo biopsies were performed at blastocyst stage (day 5/ 6). After the trophoectoderm biopsy, all embryos were vitrified. Main outcome was segmental aneuploidy rate among different male age groups (<41 y.o./ ≥40 y.o.). Lineal regression analysis was used for continuous parameters and Chi square was used for categorical variables. Main results and the role of chance Descriptive parameters showed no differences, particularly in sperm concentration (43.2±15.6 vs. 40.1±14.3) and sperm motility (27.3±13.7 vs. 29.7±14.8). Lineal regression analysis showed no significant differences in segmental aneuploidy rate (p = 0.542). Statistical model showed no significant differences among male age groups (1.28% (0.76-2.01) vs 1.10% (0.69-1.66) p = 0.542). Limitations, reasons for caution The study has been conducted in three different clinics, there could be some unknown cofounding factors. Wider implications of the findings Male age don’t have an impact on the embryo segmental aneuploidy rate in young/donors’ oocyte Trial registration number Not applicable