P-700: Effect of cilostamide and forskolin on gap junctional communicaton between human oocyte and cumulus cells and oocytes competence during in vitro maturation

Abstract

In-vitro matured oocytes are found to show lower competence than in-vivo matured oocytes due to cytoplasmic and nuclear maturation asynchrony. Delayed nuclear maturation and prolonged contact with cumulus cells have been reported to allow prolonged cytoplasmic differentiation and therefore improve oocyte developmental potential. which is synthesized in Cumulus cells by adenylate cyclase and rapidly traverses into the oocyte via gap junction between cumulus cells and oocytes. However, ooplasm cAMP can also be rapidly degraded by the very active oocyte phosphodiesterase (PDE) 3.