P-63 Effects of alternative splicing and nonsense-mediated mRNA decay on the expression of a variant of GM-CSF receptor beta chain in myelodysplastic syndromes

Abstract

mononuclear cells from 20 normal bone marrow samples. 21 patients with MDS at different stages and 32 cases of acute leukemia by using a polymrase chain reactionenzyme linked immuno-sorben assay (PCR-ELISA) kit. The TA in normal bone marrow cells was in the range of 0 to 0.3 units (U) with a mean of 0.11±0.08U. In leukemic cells, the TA was ranging from 0 to 0.96U with a mean value of 0.42±0.26U, which was significantly different from that in normal bone marrow (BM) (P < 0.01). In case of MDS, the average level of TA was 0.27±0.19 U (ranging from 0 to 0.97 U) (P < 0.05). Particularly, the MDS high-risk subgroup exhibited a significantly higher activity of telomerase (P < 0.05). In comparison with INT-1 and INT-2 subgroups in MDS patients based on international prognostic scoring system (IPSS). The abnormality in cell karyotype was not correlated with TA. So, The BM cells from MDS patients display a moderate TA among which the high risk MDS subgroup with a poor prognostic IPSS score exhibited marked higher TA.