P-387 - PPARγ regulation improved RAS/ NO/ ADMA signaling and antioxidant responce in pathophysiology of young hypertensive rats

Abstract

PPARγ plays an important role in kidney physiology of cardiovascular system, contributes in hypertension and signaling pathways. In our study we focused on the effect of PPARγ agonist pioglitazone (PIO) on the RAS, redox regulation, NOS activities, redox regulation and ADMA levels in two models of young borderline hypertensive (BHR) and spontaneously hypertensive (SHR) rats. Gene expression was detected by qRT-PCR. Protein level was determined using Western blot analysis. Superoxide dismutase (SOD) and nitric oxide synthase (NOS) activities was determined spectrophotometrically and with radioactive method. ADMA levels has been analysed by HPLC method with fluorescence detection. PIO decreased (in BHR) and slowed blood pressure development (in SHR). Gene and protein expressions of NOS isoforms and total NOS activities was improved in both experimental rats and affected ADMA changes. In AT1R/NADPH - oxidase pathway the treatment was not significantly influenced, but mRNA expression in Mas, AT2R receptors and redox regulation (Nrf2 and SOD isoforms) was up-regulated. The largest effect of PPARγ has been observed in SOD1, SOD3 and Mas receptor genes in different way: SOD1 was increased in SHR and Mas receptor in BHR rats.