P-352 - Nitric oxide-releasing porous materials and their potential regulation of in-vitro biological functions

Abstract

Nitric oxide (NO) has innumerable physiological and pathophysiological functions and its exogenous supplementation is an excellent alternative to conventional therapies. Nanoporous materials proved to be potential vehicles with high NO storage capacity, however studies concerning their toxicity, stability and their effectiveness to regulate biological processes with the delivery of NO are still very limited. In this work, potential NO-releasing titanosilicates (ETS-4 type and ETS-10 type) and clay-based materials were evaluated in terms of toxicity, stability and NO release under biological conditions. ETS-4 demonstrated to be the most promising material, combining good biocompatibility, stability and slow NO release. ETS-10 and ETAS-10 showed the best biocompatibility and, in the case of clay-based materials, CoOs is the less toxic and the one that releases higher NO. The potential of these new NO donors to regulate biological functions was evaluated by controlling the mitochondrial respiration and the cell migration, showing very encouraging results never reported before with porous NO-loaded materials. The NO-loaded ETS-4 actively regulated cells O2 consumption and decreases the wound area more 8% in 6 h than the controls, in an extremely dependent material concentration way.