P-32 * EFFECTS OF INTESTINAL MOTILITY ON ETHANOL ABSORPTION IN SMALL INTESTINE

Abstract

Administration of caffeine and Ryokucha Saponin markedly decreases the metabolic rate of ethanol. The effect of caffeine and Ryokucha Saponin on ethanol absorption in small intestine was investigated. Sample animals were used male Wistar rats (6 week). The rate of intestinal absorption of ethanol was measured using the small intestine of rats according to Creine and Wilson method ( J Appl Physiol 12 : 145–146, 1958). The solution which transmitted small intestine was measured for ethanol levels at 0 to 30 minutes in presence or absence of caffeine or Ryokucha Saponin with ethanol solution. Contractile activity of the intestine was investigated by Magnus apparatus. A small intestine was removed from rats and were placed in a Tyrode solution. The opposite end of the tissue was secured to a sensitive strain gauge and tension measurement were recorded on a kymographion. The rate of intestinal absorption of ethanol was found that the increase in the ethanol absorption is depending on time. In the presence of caffeine and Ryokucha Saponin reduced ethanol absorption about over 10% of control. Caffeine and Ryokucha Saponin inhibits intestinal motility. Based on these results, we speculate that intestinal motility by increase and decrease were augment and suppres s alcohol absorption.